Forward Larissa Crummer have been ruled out the 2016 AFC Women's Olympic Qualifiers in Osaka, Japan.

The 20 year old sustained a knee injury during Australia's final training match against an Illawarra Stingrays Select squad last night in Wollongong.

“Larissa (Crummer) suffered a knock to her knee going for a header during the first half of our training game last night and after scans this morning and consultation with the medical staff, unfortunately she won’t be available for the qualifying tournament,” said Matildas Head Coach Alen Stajcic.

“It’s really disappointing news for Larissa because she had a great W-League season and has been looking really good at training leading up to this tournament."

[MORE: Stajcic names 20 player squad]

The W-League's top goalscorer in Season 8, the Melbourne City striker will be replaced by Canberra United forward Ashleigh Sykes.

Sykes, who was a part of the Matildas squad for the 2015 FIFA Women's World Cup, was one the leading forwards and led Canberra United in goals.

“Ash Sykes will now come into the squad and with the great form she showed in the W-League, we’re confident she will add another dimension to our attack.”

The Olympic qualifying tournament will see the Matildas’ play five matches in 10 days, the first being a blockbuster against FIFA Women’s World Cup 2015 Finalists and tournament hosts Japan on 29 February.

The top two teams will qualify for the 2016 Rio de Janeiro Olympic Games Women’s Football Tournament.

Matildas squad – Women’s Olympic Football Tournament Rio 2016

Goalkeepers: Lydia Williams, Mackenzie Arnold, Casey Dumont

Defenders: Clare Polkinghorne, Laura Alleway, Steph Catley, Alanna Kennedy, Caitlin Cooper

Midfielders: Elise Kellond-Knight, Emily van Egmond, Tameka Butt, Katrina Gorry, Aivi Luik, Chloe Logarzo

Forwards: Ashleigh Sykes, Lisa De Vanna, Kyah Simon, Michelle Heyman, Emily Gielnik