Western Bulldogs and Carlton will hold the first AFLW Pride match in their round four clash at VU Whitten Oval.
The announcement this morning was made at the Pride of our Footscray, a community-owner LGTBQI bar in Footscray in Melbourne.
Players will wear specially-designed guernseys for the match.
This has not been finalised.
Hannah Scott and Bri Davey attended the launch.
"We want to teach young people how to be proud of who they are,' Scott said.
"I'm really proud to be a part of something like this and I couldn't be prouder to be a Bulldog.
"It means quite a lot to me, obviously I'm out aswell so it means a lot to my partner too. For the Bulldogs and Carlton to back this, I'm so proud to be a part of the Western Bulldogs," she said.
Western Bulldogs CEO Ameet Bains told AFL.com.au that the game was a natural fit.
"As a club, there had been a number of internal conversations that have been taking place over the last 12 months since the competition has started, around how the club can play a more proactive role in promoting gender diversity and supporting the LGTBQI community," he said.
"There's been some great support in the men's competition for this concept, but to be able to do it in the AFL Women's and celebrating a lot more than what you see on the surface is fantastic."
While Carlton's inclusion to celebrate the LGBTI community comes after the club released a statement in September last year stating "the Club encourages all of its people to have their say in this important national vote" in the marriage equality postal survey.
However, this isn't the first Pride game in the sport as Sydney Swans and St Kilda played in a Pride match in August 2016 and the second edition took place last season.
The Pride Game will be broadcast nationally on Fox Footy, streamed through the AFL website and AFLW app and broadcast on Joy 94.9 FM.