The Matildas will be missing key midfielder Elise Kellond-Knight when they play their final World Cup warm-up match with the Netherlands.
The Matildas' preparation for next month's World Cup kicks up another notch this weekend as they face the Netherlands in their last hit-out before their French campaign.
Worryingly, Australia will go without midfield stalwart Elise Kellond-Knight or defender Laura Alleway for the clash.
Both missed training on Friday (AEST) after emerging from their training camp in Antalya, Turkey, with minor issues, and won't be risked in Eindhoven.
"They won't be playing any part in tomorrow's game," coach Ante Milicic said.
"It's more more like muscle soreness (for Kellond-Knight) ... hopefully she'll be OK in a couple of days.
"Laura has been going well but had a training ground knock (on her foot). She's already moving better again."
Kellond-Knight, the fulcrum of Australia's midfield, now faces a race against time to be fit for Australia's World Cup opener with Italy on June 9.
Aivi Luik and Tameka Yallop are being considered as midfield replacements for Kellond-Knight in the clash against the European champions on Sunday morning (AEST).
Milicic is likely to field a strong side for the outing as he firms up his thoughts for the title assault.
The meeting with the Netherlands, ranked by FIFA as the eighth-best team in the world, is an ideal match-up.
In Vivianne Miedema and Lieke Mertens, they have a superb strike force likely to put the Matildas defence to the test.
Miedema, just 22 but with 57 international goals, won the English Super League's golden boot after a stellar campaign with title-winners Arsenal.
Mertens was awarded the title of best player in the world in 2017 after Holland's continental triumph.
The Matildas last met the Netherlands at the 2017 Algarve Cup, with Australia emerging 3-2 winners.
Emily Gielnik scored an early brace before Alanna Kennedy powered home a header that would prove to be the winner.
"They're a dark horse to do well in France and they're a great test for us," Kennedy said.
The outing is important for Australia to build match fitness, and to regain any lost momentum from their most recent outing; a 5-3 loss to world champions USA.
Kellond-Knight said the Matildas would face stiff opposition in Miedema.
"She's quality. A great player. They've got a lot of threat up front," she said.
"In terms of women's football they're a great nation and they've got superb support, there will probably be 30,000 there for the game."
Kick-off is at 2:15am on Sunday (AEST); 6:15pm local time on Saturday.