Australia will be without Charlotte Caslick, Sharni Williams and Ellia Green heading into the Kitakyushu Sevens.
It will be their first Series post-Tim Walsh with assistant John Manenti the interim coach for the rest of the season.
Caslick suffered a head knock in the Commonwealth Games gold medal match against New Zealand, while Williams injured her ankle on the opening day and Green has aggravated a knee injury.
Page McGregor, Lauren Brown and Yasmin Meakes come into the squad for the third leg of the Series as Australia look to maintain their World Series lead.
“Our whole focus will be on this next challenge ahead of us and such is the nature of Rugby Sevens you have to get back up very quickly for the next round of the HSBC Sevens World Series," Manenti said.
“We have some new players coming into the squad who will bring some enthusiasm and energy to the team with Page, Yasmin and Lauren now getting their opportunity," he said.